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| Summarize the differences between the two possible culprits in the attached chart using the information at the above websites |
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E. coli (strain O157) |
Salmonella |
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Which illness is caused by this organism? |
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No Answer |
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How is the infection spread? |
No Answer |
No Answer |
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What are the main pathogenic types of the bacteria? |
No Answer |
No Answer |
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Which type is the likely cause of Dan's food-borne illness? |
No Answer |
No Answer |
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What are the symptoms of the infections? |
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No Answer |
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Main toxins |
No Answer |
No Answer |
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When does symptom onset occur? |
No Answer |
No Answer |
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How can the infection be diagnosed?
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No Answer |
No Answer |
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How can the infection be treated? |
No Answer |
No Answer |
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Can the bacteria be treated by antibiotics? |
No Answer |
No Answer |
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What are possible complications of the illness? |
No Answer |
No Answer |
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1.Read the news report and write about five ways (five food agents) in which one can contract an E. coli (strain O157) infection. |
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2. How can we produce new proteins substances by inserting genes to the bacterial genome?
What are the main steps of recombiant proteins production? |
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No Answer |
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3. Look st these
sites
and write down five different substances
that are made by E.coly using biochemical known-how.
Write about the uses of those subsatances. |
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No Answer |
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4. If you were to sequence a microorganism's entire genome, in principle, what steps would you take? How would you connect all of the information you obtained, and how would you try to make sense of it (for example, find out where the genes are and what they are likely to code for)?
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No Answer |
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5. One of the challenges of combinatorial mutagenesis is the selection of molecules with
desired properties. This is particularly problematic if degeneracy has been introduced at a
large number of codons
simultaneously, resulting in a very low expected
frequency of those molecules. Can you think of
procedures you could use (for example, addition of extra steps) to boost the frequency of the
desired molecules?
No Answer |
 Genomic indexing of Salmonella and E.coli |
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Calculate the rate of survival (%) in the four groups of mice.
Why do you need to calculate the survival rate (%) of the mice rather than just looking at the numbers?
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No Answer |
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2. Draw a graph to show the survival rate in each mouse group.
Why are groups C and D important?
What are your conclusions about the best way to vaccinate the mice? |
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No Answer |
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| 3. Draw a graph to show the effect of time (days) on average antibody production in the four groups of mice. |
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No Answer |
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| 4. Describe in words the effect of time (days) on average antibody production in the four groups of mice. |
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No Answer |
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| 5. Explain the difference between the antigenic reaction in group B on days 35 and 42. |
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No Answer |
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| 6. What causes the rise in antibody titer in groups A and B on day 74? |
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No Answer |
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7. What kind of vaccination regime do you recommend? |
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No Answer |
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8. WHY do scientists spend a lot of effort on developing vaccines for animals that aren't affected by the bacteria? |
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No Answer |
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1. Which E. coli gene/genes have been inserted into plant cells? |
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No Answer |
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| 2. Which E. coli gene/genes have been inserted into plant cells? |
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No Answer |
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| 3. Why were these gene/genes chosen?
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No Answer |
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| 4. HOW is the immunity developed?
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No Answer |
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| 5. What are the advantages of making vaccine-containing foods?
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No Answer |
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| 6.What are the possible dangers of using vaccine-containing foods?
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No Answer |
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